Dengue: Methods and Protocols by Radhakrishnan Padmanabhan, Subhash G. Vasudevan

Dengue: Methods and Protocols by Radhakrishnan Padmanabhan, Subhash G. Vasudevan

By Radhakrishnan Padmanabhan, Subhash G. Vasudevan

Infection via flaviviruses comparable to dengue virus serotypes (DENV 1-4), jap encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) effect hundreds of thousands of lives and reason tens of hundreds of thousands of mortalities every year. Dengue is a world public wellbeing and fitness emergency in particular considering there's no preventative vaccine or antiviral remedy for dengue sickness. Dengue: equipment and Protocols deals the expanding variety of dengue researchers a one-stop protocol e-book with options compiled from the best laboratories engaged on dengue. Chapters disguise themes akin to dengue virus isolation from scientific samples, quantification of human antibodies opposed to the virus, assays to quantify the virus debris, the generally used mouse version to check dengue pathogenesis, vaccine and antiviral efficacies. Written within the winning Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, with no trouble reproducible protocols and notes on troubleshooting and warding off identified pitfalls.

Authoritative and simply obtainable, Dengue: tools and Protocols seeks to serve either execs and newbies with its well-honed methodologies on dengue research.

Show description

Read Online or Download Dengue: Methods and Protocols PDF

Similar diagnostics & labs books

Irritable Bowel Syndrome: Pocketbook (Pocketbooks)

Irritable bowel syndrome has emerged from relative obscurity to develop into extensively recognized because the reason for enormous morbidity, aid in caliber of existence and prices to sufferers and healthiness companies. This assessment of the present kingdom of information of the situation will act as a prepared resource of connection with either generalists and experts and also will widen the reader's realizing of a few of the patient's adventure of sensible somatic issues.

Malaria Methods and Protocols (Methods in Molecular Medicine)

Across the world revered scientists and clinicians describe in step by step element their most valuable traditional and state-of-the-art thoughts for the examine of malaria. components lined comprise medical and laboratory analysis and typing, animal types, molecular biology, immunology, mobilephone biology, vaccinology, laboratory versions, and box purposes.

Fifty neurological cases from the National Hospital

Using the wealth of fabric which has been awarded by means of clinicians on the Grand Rounds on the nationwide health center for Neurology and Neurosurgery through the years, the editors have chosen fifty circumstances on which advisor neurologists at this recognized establishment have supplied their specialist reviews each one case is gifted within the similar layout: historical past, exam, investigations, prognosis, remark by means of a professional from the clinic and illustrations

High Throughput Screening: Methods and Protocols

This 3rd version quantity expands at the prior variations either via featuring extra specified protocols for the concepts defined within the first and moment variants of excessive Throughput Screening: equipment and Protocols and by way of protecting vital new systems. the 1st bankruptcy of this ebook presents an summary of significant assay improvement innovations, whereas the remainder of the chapters element tips to enhance and execute monitors at no matter what throughput the consumer wishes.

Extra info for Dengue: Methods and Protocols

Example text

Neutralization assay using Vero cells: Dilute a prior titrated virus stock to 50 μL/well using 2 h infection medium.  1, transfer 50 μL of diluted virus to each appropriate well in a U-bottom 96-well plate. Transfer 50 μL of diluted monoclonal antibody or human serum into the appropriate wells in the plate, mix by pipetting and incubate plate at 37 °C in an incubator containing 5 % CO2 for 1 h. After the 1 h incubation, aspirate medium from Vero cell-coated wells, transfer the antibody-­virus mixture to the aspirated wells, and incubate for 2 h at 37 °C in an incubator containing 5 % CO2.

Gently add 5 mL of 24 % (w/v) sucrose to the bottom of the suspension using a Pasteur pipette. Do not mix the two solutions. 5 h. Invert the tube to remove the sucrose and wick off the excess solution. Add 500 μL NTE buffer to cover the pellet. This is kept overnight at 4 °C to allow the pellet to soften (see Note 8). 5 mL Eppendorf tube. Centrifuge on a benchtop centrifuge at 16,000 × g for 2 min. Transfer the supernatant into a new Eppendorf tube and repeat the centrifugation process. 7. Prepare a potassium tartrate-glycerol gradient ranging from 10 to 30 % in an ultra-clear tube suitable for the specific ultracentrifuge rotor.

16. Let slides air-dry completely. 17. Place a drop of mounting fluid onto the slide and gently lay down the coverslip. 18. View slides using a fluorescent microscope (see Note 12). 19. Record the number of infected mosquitoes for each dilution. 20. Calculate virus titers using the method of Reed and Muench and express the titers in terms of the dose that infects 50 % of the mosquitoes inoculated (MID50 per ml). Mosquito Inoculation Technique 4 23 Notes 1. The needles can be pulled using a simple alcohol lamp if a needle puller is not available.

Download PDF sample

Rated 4.91 of 5 – based on 43 votes
Comments are closed.